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In vitro selection system for the isolation of bacteriophage promoters was developed. Employing the system, one round of the selection achieved ∼20-30 fold enrichment of an active promoter from mixtures with an inactive one. In addition, active T7 promoter variants were successfully selected from a library with six randomized bases. Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.


Shoji Ohuchi. In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer. Journal of bioscience and bioengineering. 2012 Jul;114(1):110-2

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PMID: 22560719

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