In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer.

Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. sohuchi@ims.u-tokyo.ac.jp

Journal of bioscience and bioengineering 2012 Jul

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In vitro selection system for the isolation of bacteriophage promoters was developed. Employing the system, one round of the selection achieved ∼20-30 fold enrichment of an active promoter from mixtures with an inactive one. In addition, active T7 promoter variants were successfully selected from a library with six randomized bases. Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Citation

Shoji Ohuchi. In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer. Journal of bioscience and bioengineering. 2012 Jul;114(1):110-2