Immunohistochemistry and detection of proliferating cells by BrdU.

Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, Chrissa Kioussi

Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.

Methods in molecular biology (Clifton, N.J.) 2012

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Immunohistochemistry is a classic technique used for the localization of antigenic target molecules in -tissue. The method exploits the principle that the target antigen is recognized by specific antibody and is visualized using different detection systems. The subject of this chapter is simultaneous immunohistochemical detection of protein antigens and proliferation marker BrdU in the developing tooth.

Citation

Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, Chrissa Kioussi. Immunohistochemistry and detection of proliferating cells by BrdU. Methods in molecular biology (Clifton, N.J.). 2012;887:33-9