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Detection of apoptosis by TUNEL assay.

Kateryna Kyrylkova, Sergiy Kyryachenko, Mark Leid, Chrissa Kioussi

Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.

Methods in molecular biology (Clifton, N.J.) 2012


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    Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.

    Citation

    Kateryna Kyrylkova, Sergiy Kyryachenko, Mark Leid, Chrissa Kioussi. Detection of apoptosis by TUNEL assay. Methods in molecular biology (Clifton, N.J.). 2012;887:41-7

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    PMID: 22566045

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