Establishment of detection system of CNVs HMGR, SQS1, beta-AS synthase gene of Glycyrrhiza uralensis].

Ying Liu, Dongji Liu, Chunsheng Liu

School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. liuyliwd@vip.sina.com.cn

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 2012 Feb

filter terms:

To establish a stabilized and reliable detection system of CNVs of HMGR, SQS1, beta-AS gene of Glycyrrhiza uralensis. Real time PCR was used to detect the CNVs of HMGR, SQS1, beta-AS gene of G. uralensis. In the quantitative detection experiments of HMGR, SQS1, beta-AS gene of G. uralensis, the change of value of C(t) was 25.82-25.88, 29.01-29. 08, 15.52-15.56, 19.06-19.08 respectively, the alue of SD was 0.033, 0.032, 0.024, 0.011 respectively, and the value of CV was 0.12%, 0.22%, 0.16%, 0.06% respectively. The repeatability of detection system of Real time PCR was stabilized and reliable, and the method could be used to detect the CNVs of HMGR, SQS1, beta-AS gene of G. uralensis.

Citation

Ying Liu, Dongji Liu, Chunsheng Liu. Establishment of detection system of CNVs HMGR, SQS1, beta-AS synthase gene of Glycyrrhiza uralensis]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 2012 Feb;37(3):283-7