Structural analysis of the O-polysaccharide of the lipopolysaccharide from Azospirillum brasilense Jm6B2 containing 3-O-methyl-D-rhamnose (D-acofriose).

Alevtina S Boyko, Andrey S Dmitrenok, Yuliya P Fedonenko, Evelina L Zdorovenko, Svetlana A Konnova, Yuriy A Knirel, Vladimir V Ignatov

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.

Carbohydrate research 2012 Jul 1

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Two types of neutral O-polysaccharides were obtained by mild acid degradation of the lipopolysaccharide isolated by phenol-water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense Jm6B2. The following structure of the major O-polysaccharide was established by composition and methylation (ethylation) analyses, Smith degradation, and 1D and 2D (1)H and (13)C NMR spectroscopy: [structure: see text] where a non-stoichiometric (~60%) 3-O-methylation of D-rhamnose is indicated by italics. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Alevtina S Boyko, Andrey S Dmitrenok, Yuliya P Fedonenko, Evelina L Zdorovenko, Svetlana A Konnova, Yuriy A Knirel, Vladimir V Ignatov. Structural analysis of the O-polysaccharide of the lipopolysaccharide from Azospirillum brasilense Jm6B2 containing 3-O-methyl-D-rhamnose (D-acofriose). Carbohydrate research. 2012 Jul 1;355:92-5