Human cells: new platform for recombinant therapeutic protein production.

Kamilla Swiech, Virgínia Picanço-Castro, Dimas Tadeu Covas

Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. kamilla@fcfrp.usp.br

Protein expression and purification 2012 Jul

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The demand for recombinant therapeutic proteins is significantly increasing. There is a constant need to improve the existing expression systems, and also developing novel approaches to face the therapeutic proteins demands. Human cell lines have emerged as a new and powerful alternative for the production of human therapeutic proteins because this expression system is expected to produce recombinant proteins with post translation modifications more similar to their natural counterpart and reduce the potential immunogenic reactions against nonhuman epitopes. Currently, little information about the cultivation of human cells for the production of biopharmaceuticals is available. These cells have shown efficient production in laboratory scale and represent an important tool for the pharmaceutical industry. This review presents the cell lines available for large-scale recombinant proteins production and evaluates critically the advantages of this expression system in comparison with other expression systems for recombinant therapeutic protein production. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Kamilla Swiech, Virgínia Picanço-Castro, Dimas Tadeu Covas. Human cells: new platform for recombinant therapeutic protein production. Protein expression and purification. 2012 Jul;84(1):147-53