X-linked inhibitor of apoptosis protein mediates neddylation by itself but does not function as a NEDD8-E3 ligase for caspase-7.

Taiki Nagano, Toshiaki Hashimoto, Akio Nakashima, Ushio Kikkawa, Shinji Kamada

Biosignal Research Center, Kobe University, Kobe 657-8501, Japan.

FEBS letters 2012 Jun 4

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X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a bona fide target for NEDD8. Here we showed that no neddylation of caspase-7 but that of XIAP itself was observed under the conditions in which caspase-7 was modified with ubiquitin. These results reveal that XIAP does not function as a NEDD8-E3 ligase for caspase-7 in vivo. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Taiki Nagano, Toshiaki Hashimoto, Akio Nakashima, Ushio Kikkawa, Shinji Kamada. X-linked inhibitor of apoptosis protein mediates neddylation by itself but does not function as a NEDD8-E3 ligase for caspase-7. FEBS letters. 2012 Jun 4;586(11):1612-6