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Detection of glycoproteins in polyacrylamide gels using Pro-Q Emerald 300 dye, a fluorescent periodate Schiff-base stain.
Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. Padmaja-Mehta@omrf.org
Methods in molecular biology (Clifton, N.J.) 2012Sizes of these terms reflect their relevance to your search.
Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. With this dye, it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. This dye is more sensitive than the standard periodic acid Schiff's base which uses acidic fuchsin dye.
Citation
Padmaja Mehta-D'souza. Detection of glycoproteins in polyacrylamide gels using Pro-Q Emerald 300 dye, a fluorescent periodate Schiff-base stain. Methods in molecular biology (Clifton, N.J.). 2012;869:561-6
PMID: 22585521
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