Production of truncated protein by the crosslink formation of mRNA with 2'-OMe oligoribonucleotide containing 2-amino-6-vinylpurine.

Shinya Hagihara, Shuhei Kusano, Wei-Chen Lin, Xiao-Guang Chao, Tsuneaki Hori, Shuhei Imoto, Fumi Nagatsugi

Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Miyagi 980-8577, Japan.

Bioorganic & medicinal chemistry letters 2012 Jun 15

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The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Shinya Hagihara, Shuhei Kusano, Wei-Chen Lin, Xiao-Guang Chao, Tsuneaki Hori, Shuhei Imoto, Fumi Nagatsugi. Production of truncated protein by the crosslink formation of mRNA with 2'-OMe oligoribonucleotide containing 2-amino-6-vinylpurine. Bioorganic & medicinal chemistry letters. 2012 Jun 15;22(12):3870-2