Production of truncated protein by the crosslink formation of mRNA with 2'-OMe oligoribonucleotide containing 2-amino-6-vinylpurine.
Shinya Hagihara, Shuhei Kusano, Wei-Chen Lin, Xiao-Guang Chao, Tsuneaki Hori, Shuhei Imoto, Fumi Nagatsugi
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Miyagi 980-8577, Japan.
Bioorganic & medicinal chemistry letters 2012 Jun 15The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position. Copyright © 2012 Elsevier Ltd. All rights reserved.
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Shinya Hagihara, Shuhei Kusano, Wei-Chen Lin, Xiao-Guang Chao, Tsuneaki Hori, Shuhei Imoto, Fumi Nagatsugi. Production of truncated protein by the crosslink formation of mRNA with 2'-OMe oligoribonucleotide containing 2-amino-6-vinylpurine. Bioorganic & medicinal chemistry letters. 2012 Jun 15;22(12):3870-2
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PMID: 22613261
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