Designing quantum rods for optimized energy transfer with firefly luciferase enzymes.
Rabeka Alam, Danielle M Fontaine, Bruce R Branchini, Mathew M Maye
Department of Chemistry, Syracuse University, Syracuse, New York 13244, United States.
Nano letters 2012 Jun 13The bioluminescence resonance energy transfer (BRET) between firefly luciferase from Photinus pyralis (Ppy) with core/shell semiconductive quantum rods (QRs) has been studied as a function of QR aspect ratio and internal microstructure. The QRs were found to be ideal energy acceptors, and Ppy-to-core distances were optimized using rod-in-rod microstructures that were achieved by the synthetic control of rod morphology, surface chemistry, and Ppy:QR loading. The BRET ratios of >44 measured are the highest efficiencies to date.
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Rabeka Alam, Danielle M Fontaine, Bruce R Branchini, Mathew M Maye. Designing quantum rods for optimized energy transfer with firefly luciferase enzymes. Nano letters. 2012 Jun 13;12(6):3251-6
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PMID: 22620681
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