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The bioluminescence resonance energy transfer (BRET) between firefly luciferase from Photinus pyralis (Ppy) with core/shell semiconductive quantum rods (QRs) has been studied as a function of QR aspect ratio and internal microstructure. The QRs were found to be ideal energy acceptors, and Ppy-to-core distances were optimized using rod-in-rod microstructures that were achieved by the synthetic control of rod morphology, surface chemistry, and Ppy:QR loading. The BRET ratios of >44 measured are the highest efficiencies to date.


Rabeka Alam, Danielle M Fontaine, Bruce R Branchini, Mathew M Maye. Designing quantum rods for optimized energy transfer with firefly luciferase enzymes. Nano letters. 2012 Jun 13;12(6):3251-6

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PMID: 22620681

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