Changes of faecal microbiota in patients with Crohn's disease treated with an elemental diet and total parenteral nutrition.

Hisashi Shiga, Takayuki Kajiura, Junko Shinozaki, Sho Takagi, Yoshitaka Kinouchi, Seiichi Takahashi, Kenichi Negoro, Katsuya Endo, Yoichi Kakuta, Manabu Suzuki, Tooru Shimosegawa

Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai 980-8574, Japan. shiga@med.tohoku.ac.jp

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2012 Sep

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Intestinal microbiota contributes to the pathogenesis of Crohn's disease. Elemental diet and total parenteral nutrition are effective therapies for Crohn's disease; however, changes of microbiota as a result of both treatments have not been fully elucidated. To elucidate changes of faecal microbiota in Crohn's disease patients treated with elemental diet and total parenteral nutrition. Stool samples were collected from 33 active Crohn's disease patients and 17 healthy subjects, and recollected after elemental diet (8 patients) and total parenteral nutrition (9 patients). Terminal restriction fragment length polymorphism analysis of bacterial 16srDNA was performed to evaluate the whole microbiota. Specific quantitative PCR was then used to determine populations of predominant bacterial groups. In Crohn's disease patients, the number of terminal restriction fragments, which reflects bacterial species, was significantly lower. Populations of total bacteria and Bifidobacterium were significantly lower and the ratio of Enterococcus was higher. The number of terminal restriction fragments was significantly decreased after total parenteral nutrition, but not after elemental diet. Population of Bacteroides fragilis significantly decreased after elemental diet, while population of Enterococcus significantly increased after total parenteral nutrition. Faecal microbiota in Crohn's disease patients was markedly different from healthy subjects. Species diversity was reduced by total parenteral nutrition, but not by elemental diet. Copyright © 2012 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

Citation

Hisashi Shiga, Takayuki Kajiura, Junko Shinozaki, Sho Takagi, Yoshitaka Kinouchi, Seiichi Takahashi, Kenichi Negoro, Katsuya Endo, Yoichi Kakuta, Manabu Suzuki, Tooru Shimosegawa. Changes of faecal microbiota in patients with Crohn's disease treated with an elemental diet and total parenteral nutrition. Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver. 2012 Sep;44(9):736-42