Structural and biochemical characterization of native and recombinant single insulin-like growth factor-binding domain protein (SIBD-1) from the Central American hunting spider Cupiennius salei (Ctenidae).

Christian Trachsel, Christine Widmer, Urs Kämpfer, Claudia Bühr, Tommy Baumann, Lucia Kuhn-Nentwig, Stefan Schürch, Johann Schaller, Ulrich Baumann

Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland.

Proteins 2012 Aug

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Cupiennius salei single insulin-like growth factor binding domain protein (SIBD-1) is an 8.6 kDa Cys-, Pro-, and Gly-rich protein, discovered in the hemocytes of the Central American hunting spider Cupiennius salei. SIBD-1 exhibits high sequence similarity to the N-terminal domain of the insulin-like growth factor-binding protein superfamily and has been reported to play an important role in the spider's immune system. Here, the recombinant expression and the elucidation of the three-dimensional structure of recombinant SIBD-1 and the characterization of the sugar moiety at Thr2 of native SIBD-1 is described in detail. Copyright © 2012 Wiley Periodicals, Inc.

Citation

Christian Trachsel, Christine Widmer, Urs Kämpfer, Claudia Bühr, Tommy Baumann, Lucia Kuhn-Nentwig, Stefan Schürch, Johann Schaller, Ulrich Baumann. Structural and biochemical characterization of native and recombinant single insulin-like growth factor-binding domain protein (SIBD-1) from the Central American hunting spider Cupiennius salei (Ctenidae). Proteins. 2012 Aug;80(9):2323-9