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The Ca(2+)-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoH(V83F), was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoH(V83F) mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH. Copyright © 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Citation

Taísa Magnani Dinamarco, Neil Andrew Brown, Ricardo Sergio Couto de Almeida, Patrícia Alves de Castro, Marcela Savoldi, Maria Helena de Souza Goldman, Gustavo Henrique Goldman. Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase. Microbes and infection / Institut Pasteur. 2012 Sep;14(11):922-9

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PMID: 22634424

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