Biotechnological utilization of biodiesel-derived glycerol for the production of ribonucleotides and microbial biomass.

Juan Daniel Rivaldi, Boutros Fouad Sarrouh, Ricardo de Freitas Branco, Ismael Maciel de Mancilha, Silvio Silvério da Silva

Departamento de Biotecnologia-LOT, Escola de Engenharia de Lorena, Universidade de São Paulo, Estrada Municipal do Campinho, PO Box 116, 12602810 Lorena, São Paulo, Brazil.

Applied biochemistry and biotechnology 2012 Aug

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Ten yeast strains were evaluated concerning their capabilities to assimilate biodiesel-derived glycerol in batch cultivation. The influence of glycerol concentration, temperature, pH and yeast extract concentration on biomass production was studied for the yeast selected. Further, the effect of agitation on glycerol utilization by the yeast Hansenula anomala was also studied. The yeast H. anomala CCT 2648 showed the highest biomass yield (0.30 g g(-1)) and productivity (0.19 g L(-1) h(-1)). Citric acid, succinic acid, acetic acid and ethanol were found as the main metabolites produced. The increase of yeast extract concentration from 1 to 3 g L(-1) resulted in high biomass production. The highest biomass concentration (21 g L(-1)), yield (0.45 g g(-1)) and productivity (0.31 g L(-1) h(-1)), as well as ribonucleotide production (13.13 mg g(-1)), were observed at 700 rpm and 0.5 vvm. These results demonstrated that glycerol from biodiesel production process showed to be a feasible substrate for producing biomass and ribonucleotides by yeast species.

Citation

Juan Daniel Rivaldi, Boutros Fouad Sarrouh, Ricardo de Freitas Branco, Ismael Maciel de Mancilha, Silvio Silvério da Silva. Biotechnological utilization of biodiesel-derived glycerol for the production of ribonucleotides and microbial biomass. Applied biochemistry and biotechnology. 2012 Aug;167(7):2054-67