Effects of eating disorders on oral fungal diversity.

The eating disorders anorexia and bulimia nervosa can cause several systemic and oral alterations related to poor nutrition and induced vomiting; however, the oral microflora of these patients is poorly studied. The aim of this study was to evaluate fungal microflora in the oral cavity of these patients by culture-dependent and culture-independent methods. Oral rinse samples were cultured to assess the prevalence of Candida species, and the isolates were identified by API system. Microorganism counts were compared by the Mann-Whitney test (5%). Ribotyping, a type of molecular analysis, was performed by sequencing the D1/D2 regions of 28S rRNA. Our results demonstrated that the eating disorder group showed higher oral Candida spp. prevalence with culture-dependent methods and higher species diversity with culture-independent methods. Eating disorders can lead to an increased oral Candida carriage. Culture-independent identification found greater fungal diversity than culture-dependent methods. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Graziella Nuernberg Back-Brito, Adolfo José da Mota, Luciano Ângelo de Souza Bernardes, Sílvia Satomi Takamune, Eliane de Fátima Gomes Barbosa Prado, Táki Athanássios Cordás, Ivan Balducci, Francisco Gorgônio da Nobrega, Cristiane Yumi Koga-Ito. Effects of eating disorders on oral fungal diversity. Oral surgery, oral medicine, oral pathology and oral radiology. 2012 Apr;113(4):512-7

Mesh Tags

Substances

PMID: 22668429

search → result