Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides.

Torben Højland, Rakesh N Veedu, Birte Vester, Jesper Wengel

Artificial DNA, PNA & XNA 2012 Jan-Mar

filter terms:

We describe the first enzymatic incorporation of an α-L-LNA nucleotide into an oligonucleotide. It was found that the 5'-triphosphate of α-L-LNA is a substrate for the DNA polymerases KOD, 9°N(m), Phusion and HIV RT. Three dispersed α-L-LNA thymine nucleotides can be incorporated into DNA strands by all four polymerases, but they were unable to perform consecutive incorporations of α-L-LNA nucleotides. In addition it was found that primer extension can be achieved using templates containing one α-L-LNA nucleotide.

Citation

Torben Højland, Rakesh N Veedu, Birte Vester, Jesper Wengel. Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides. Artificial DNA, PNA & XNA. 2012 Jan-Mar;3(1):14-21