Ultrasound-intensified laccase production from Trametes versicolor.

Feng Wang, An-Zhou Ma, Chen Guo, Guo-Qiang Zhuang, Chun-Zhao Liu

National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.

Ultrasonics sonochemistry 2013 Jan

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An efficient intermittent ultrasonic treatment strategy was developed to improve laccase production from Trametes versicolor mycelia cultures. The optimized strategy consisted of exposing 2-day-old mycelia cultures to 5-min ultrasonic treatments for two times with a 12-h interval at the fixed ultrasonic power and frequency (120 W, 40 kHz). After 5 days of culture, this strategy produced the highest extracellular laccase activity of 588.9 U/L among all treatments tested which was 1.8-fold greater than the control without ultrasound treatment. The ultrasonic treatment resulted in a higher pellet porosity that facilitated the mass transfer of nutrients and metabolites from the pellets to the surrounding liquid. Furthermore, the ultrasonic treatment induced the expression of the laccase gene (lcc), which correlated with a sharp increase in both extracellular and intracellular laccase activity. This is the first study to find positive effects of ultrasound on gene expression in fungal cells. These results provide a basis for understanding the stimulation of metabolite production and process intensification by ultrasonic treatment in filamentous fungal culture. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Feng Wang, An-Zhou Ma, Chen Guo, Guo-Qiang Zhuang, Chun-Zhao Liu. Ultrasound-intensified laccase production from Trametes versicolor. Ultrasonics sonochemistry. 2013 Jan;20(1):118-24