Proteomic analysis of the copper ion-induced stress response in a human embryonic carcinoma cell line.

Excessive exposure to copper, a redox-active metal, generates free radicals, which can cause cellular damage. In this study, we aim to identify the proteins that are up- or downregulated by copper exposure in human embryonic carcinoma (NCCIT) cells and to understand the mechanisms that play a role in the copper-induced stress response. After exposure to copper ions, the cells showed upregulated levels of 78 kDa glucose-regulated protein, fibrillin 1, CWC22 spliceosome-associated protein (KIAA1604), heat shock protein (HSP) 60, and HSP70, while the tumor necrosis factor receptor-associated factor 6, vimentin, 14-3-3 protein zeta, and RAC-beta (AKT2) serine/threonine protein kinase were downregulated. The GeneGo Process Networks of the proteins upregulated by copper ions were analyzed, and the 3 highest-scoring networks from the proteins upregulated by copper ions are presented here. In particular, the increased level of HSP70 in response to copper ions occurred in a dose-dependent manner, indicating that HSP70 could be a potential biomarker for copper toxicity in mammalian cells.

Citation

Dal Mu Ri Han, Mi Ran Choi, Kyoung Hwa Jung, Hyung Tae Lee, Ji Hyun Park, Takbum Ohn, Young Gyu Chai. Proteomic analysis of the copper ion-induced stress response in a human embryonic carcinoma cell line. International journal of toxicology. 2012 Jul-Aug;31(4):397-406

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PMID: 22692975

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