Zhongjiang Zhou, Haiyan Ye, Kai Cui, Xianghui Chen, Yili Liu
Department of Cardiology, Southern Medical University, Guangzhou, China. zhouzhongjiang@126.com
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2012 JunTo characterize a new alternative splicing isoform of human vascular endothelial growth factor (VEGF) gene. The total RNA was extracted from the lung tissue of a legally aborted 4-month-old fetus and amplified by RT-PCR. The amplified product was cloned into the plasmid pMD18-T and plasmid pcDNA3.1- for sequence analysis. Electrophoresis of the RT-PCR products displayed one short band for VEGF(121) (487 bp) and a long band. The latter was characterized to contain two fragments: one was normal VEGF(165) (619 bp), and the other (639 bp) had an identical nucleotide sequence to VEGF(165) with a 20 bp fragment inserted between exons 3 and 4. Sequence analysis showed that this 20-bp nucleotide was inserted from the 3' end of the third intron containing a splicing signal, thus causing shift mutation in the reading frame of VEGF gene and early appearance of the stop codon UAG in the middle of exon 4. A new alternative splicing isoform of VEGF probably exists in the lung tissue of a legally aborted human fetus, and its biological significance remains to be further investigated.
Zhongjiang Zhou, Haiyan Ye, Kai Cui, Xianghui Chen, Yili Liu. A probable novel splicing isoform of human vascular endothelial growth factor. Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 2012 Jun;32(6):755-9
PMID: 22699049
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