Production and characterization of monoclonal antibodies to the hemocytes of mud crab, Scylla serrata.

Brajendu Kumar, A Deepika, M Makesh, C S Purushothaman, K V Rajendran

Central Institute of Fisheries Education, Versova, Andheri (W), Mumbai 400 061, India.

Journal of invertebrate pathology 2012 Sep 15

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Monoclonal antibodies (MAbs) to hemocytes of mud crab, Scylla serrata, were produced by immunizing mice with formalin-fixed hemocytes. Of the six MAbs produced, two (MAb 1D11 and MAb 1A2) reacted specifically with hemocyte proteins in western blot. MAb 1A2 showed strong immunofluorescent reaction with granular hemocytes and a weak reaction with semigranular cells. However, hyaline cells did not show any reaction. The MAbs also showed strong cross-reactivity with the hemocytes of different crab species but not with other crustaceans. The MAbs produced can be used as a marker for granular hemocytes of crabs. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Brajendu Kumar, A Deepika, M Makesh, C S Purushothaman, K V Rajendran. Production and characterization of monoclonal antibodies to the hemocytes of mud crab, Scylla serrata. Journal of invertebrate pathology. 2012 Sep 15;111(1):86-9