Standardized methods for acute and semichronic toxicity tests with the copepod Acartia tonsa.

Gessica Gorbi, Marion Invidia, Federica Savorelli, Olga Faraponova, Elisabetta Giacco, Monica Cigar, Isabella Buttino, Tristano Leoni, Ermelinda Prato, Ines Lacchetti, Sandra Sei

Department of Environmental Sciences, University of Parma, Parma, Italy. gessica.gorbi@unipr.it

Environmental toxicology and chemistry / SETAC 2012 Sep

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The availability of standardized protocols for both organism culture and bioassay with ecologically relevant species is of great concern in ecotoxicology. Acartia tonsa represents an important, often dominant, member of zooplankton communities and meets all the practical criteria suggested for model species. New standardized procedures for laboratory culturing of the copepod A. tonsa and standardized methods for acute (24- and 48-h) and semichronic (7-d, static-renewal) toxicity tests with the nauplius stage are described. In both cases, eggs are the starting stage, and nauplius immobilization is the endpoint. The methods were the object of an intercomparison test involving nine laboratories, and nickel was the reference toxicant. Relative reproducibility was 24, 25, and 34% for 24-h, 48-h, and 7-d tests, respectively. Copyright © 2012 SETAC.

Citation

Gessica Gorbi, Marion Invidia, Federica Savorelli, Olga Faraponova, Elisabetta Giacco, Monica Cigar, Isabella Buttino, Tristano Leoni, Ermelinda Prato, Ines Lacchetti, Sandra Sei. Standardized methods for acute and semichronic toxicity tests with the copepod Acartia tonsa. Environmental toxicology and chemistry / SETAC. 2012 Sep;31(9):2023-8