α-Ketoglutaric acid production by Yarrowia lipolytica and its regulation.

Svetlana V Kamzolova, Maria N Chiglintseva, Julia N Lunina, Igor G Morgunov

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, Moscow region, 142290, Russia. kamzolova@rambler.ru

Applied microbiology and biotechnology 2012 Nov

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The yeast Yarrowia lipolytica VKM Y-2412 was selected as a prospective producer of α-ketoglutaric acid (KGA) from ethanol. The following peculiarities were found: (1) the intensive KGA production occurred only under the limitation of cell growth by thiamine and the excess of ethanol and nitrogen, (2) the production of KGA from ethanol required increased amount of zinc and iron ions, and (3) KGA production increased significantly with a high aeration at pH medium equal to 3.5. Under optimal conditions, the Y. lipolytica VKM Y-2412 produced up to 172 g l(-1) of KGA with the mass yield coefficient of 0.70 g g(-1).

Citation

Svetlana V Kamzolova, Maria N Chiglintseva, Julia N Lunina, Igor G Morgunov. α-Ketoglutaric acid production by Yarrowia lipolytica and its regulation. Applied microbiology and biotechnology. 2012 Nov;96(3):783-91