Correlation Engine 2.0
Clear Search sequence regions

Sizes of these terms reflect their relevance to your search.

Double-stranded RNA (dsRNA) molecules are widely found in yeasts and filamentous fungi. It has been suggested that may play important roles in the evolution of eukaryote genomes and may be a valuable tool in yeast typing. The characterization of these extrachromosomal genetic elements is usually a laborious process, especially when trying to analyze a large number of samples. In this chapter, we describe a simple method to isolate dsRNA elements from yeasts using low amounts of starting material, and their application to different Xanthophyllomyces dendrorhous strains. Furthermore, the methodologies for enzymatic and hybridization characterizations, and quantification of relative dsRNA abundance are detailed.


Marcelo Baeza, María Fernández-Lobato, Víctor Cifuentes. Isolation and characterization of extrachromosomal double-stranded RNA elements in Xanthophyllomyces dendrorhous. Methods in molecular biology (Clifton, N.J.). 2012;898:195-205

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 22711127

View Full Text