A universal platform for amplified multiplexed DNA detection based on exonuclease III-coded magnetic microparticle probes.

Ming Luo, Xia Xiang, Dongshan Xiang, Si Yang, Xinghu Ji, Zhike He

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.

Chemical communications (Cambridge, England) 2012 Jul 28

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An amplified multiplexed DNA detection biosensor has been developed, which combines the unique cleavage function of exonuclease III (Exo III) with the separating ability of magnetic microparticles (MMPs). By using different fluorophores, the multiplexed detection of DNA is demonstrated.

Citation

Ming Luo, Xia Xiang, Dongshan Xiang, Si Yang, Xinghu Ji, Zhike He. A universal platform for amplified multiplexed DNA detection based on exonuclease III-coded magnetic microparticle probes. Chemical communications (Cambridge, England). 2012 Jul 28;48(59):7416-8