Association of CFU-Mk with total colony-forming units in thawed cord blood units.

Cord blood unit (CBU) total colony-forming unit (CFU) count both pre-cryo and post-thaw has been shown to be associated with platelet (PLT) engraftment. Pre-cryo CBUs show good growth of megakaryocytic CFUs (CFU-Mk); however, CFU-Mk have rarely been studied in post-thaw CBUs. Nucleated cells (NCs) from post-thaw CB were cultured in a collagen-based assay designed to support growth of CFU-Mk. To ensure accurate counting two independent investigators evaluated four culture chambers per sample for CFU-Mk growth. Post-thaw CFU and other cellular characteristics of the CBUs were enumerated independently and compared to CFU-Mk. The post-thaw CBU total CFU count varied from 0·47 to 4·20×10(6) colonies (median, 0·99×10(6)) and total CFU-Mk count from 0·11 to 0·70×10(6) colonies (median, 0·21×10(6)). Total CFU-Mk count was closely associated with total CFU count (Spearman's Rho=0·86, P=0·0072), haemoglobinized CFU (Rho=0·86, P=0·0072) and CFU-granulocyte/macrophage (CFU-GM; Rho=0·81; P=0·0154). Total CFU-Mk count also correlated with the post-thaw total CD34+ cell count (median, 2·55×10(6); range, 1·40-12·5×10(6); Rho=0·83; P=0·0154). CFU-Mk growth was associated with total CFU, haemoglobinized CFU, CFU-GM and CD34+ cells in thawed CBUs. This study confirms the preservation of CFU-Mk potential after CB cryopreservation. © 2012 The Author(s). Vox Sanguinis © 2012 International Society of Blood Transfusion.

Citation

M Eskola, A Arvola, S Juutistenaho, K Aranko, R Kekomäki. Association of CFU-Mk with total colony-forming units in thawed cord blood units. Vox sanguinis. 2012 Nov;103(4):331-6

Mesh Tags

PMID: 22725627

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