Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae.

Sandrine Bernabeu, Laurent Poirel, Patrice Nordmann

Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K.-Bicêtre, France

Diagnostic microbiology and infectious disease 2012 Sep

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Carbapenem-hydrolyzing ß-lactamases are the most powerful ß-lactamases being able to hydrolyse almost all ß-lactams. They are mostly of the KPC, VIM, IMP, NDM, and OXA-48 type. A spectrophotometry technique based on analysis of the imipenem hydrolysis has been developed that differentiated carbapenemase- from noncarbapenemase producers. This inexpensive technique adapted to screening of carbapenemase producers may be implemented in any reference laboratory worldwide. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Sandrine Bernabeu, Laurent Poirel, Patrice Nordmann. Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae. Diagnostic microbiology and infectious disease. 2012 Sep;74(1):88-90