Simultaneous determination of hypocrellin A, hypocrellin B, and hypocrellin C by HPLC].

Ming Kong, Zhanli Chen, Zhiqi Yin, Jian Zhang

Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 2012 Jan

filter terms:

To develop a high-performance liquid chromatography (HPLC) method for simultaneous determination of hypocrellin A, hypocrellin B, and hypocrellin C. The separation was carried out on a Kromasil C18 (4.6 mm x 250 mm, 5 micrm) colum eluted with in mobile phases of water containing 0.5% glacial acetic acid and acetonitrile. The column temperature was 35 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 265 nm. The three compounds were well separated. Calibration curves of hypocrellin A, hypocrellin B, and hypocrellin C showed good linear relationship RSD > 2.0%. The average recoveries of the hypocrellin A, hypocrellin B, and hypocrellin C were 101.8%, 102.3%, 100.0%, respectively. The developed method is simple, accurate, and repeatable, and can be readily used as valid tool for the quality control of Hypocrella bambusae.

Citation

Ming Kong, Zhanli Chen, Zhiqi Yin, Jian Zhang. Simultaneous determination of hypocrellin A, hypocrellin B, and hypocrellin C by HPLC]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 2012 Jan;37(1):75-8