Correlation Engine 2.0
Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

The detection of regions of heterochromatin has been the subject of intense investigation. We investigated an adaptation of the commonly used technique by replacing the nonfluorescent dye, Giemsa, by a fluorescent one, propidium iodide. This adaptation produces greater contrast of the heterochromatic bands in metaphase chromosomes and can be especially valuable when the organisms studied possess heterochromatin that is pale and difficult to visualize. We discuss the interactions of these two dyes with DNA and the excitation of the fluorescent dye when irradiated with ultraviolet light.

Citation

R L Lui, D R Blanco, O Moreira-Filho, V P Margarido. Propidium iodide for making heterochromatin more evident in the C-banding technique. Biotechnic & histochemistry : official publication of the Biological Stain Commission. 2012 Oct;87(7):433-8

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 22747174

View Full Text