A Karpeta, K Warzecha, J Jerzak, A Ptak, E L Gregoraszczuk
Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Krakow, Poland.
Reproductive toxicology (Elmsford, N.Y.) 2012 NovThe aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism. Basal CYP1A1 and CYP2B1/2 activity increased during culture. BDE-47 had no effect on CYP1A1, however increased CYP2B1/2 activity after exposure for 6h. Basal SULT1A activity was 2.5 fold lower than that of COMT, and both proteins were stable during culture. BDE-47 increased SULT1A after exposure for 6 h, and COMT activity after exposure for 24 and 48 h. BDE-47 had no effect on the expression of all investigated enzymes. In conclusion, fast activation of CYP2B1/2 and late activation of COMT (with a very low basal SULT1A activity) indicates a possible action of locally produced hydroxylated metabolites prior to their detoxification. Copyright © 2012 Elsevier Inc. All rights reserved.
A Karpeta, K Warzecha, J Jerzak, A Ptak, E L Gregoraszczuk. Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary. Reproductive toxicology (Elmsford, N.Y.). 2012 Nov;34(3):436-42
PMID: 22750137
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