GWAS reveals new recessive loci associated with non-syndromic facial clefting.

European journal of medical genetics 2012 Oct

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We have applied a GWAS to 40 consanguineous families segregating cases of non-syndromic cleft lip with or without cleft palate (NS CL/P) (a total of 160 affected and unaffected individuals) in order to trace potential recessive loci that confer susceptibility to this common facial malformation. Pedigree-based association test (PBAT) analyses reported nominal evidence of association and linkage over SNP markers located at 11q25 (rs4937877, P = 2.7 × 10(-6)), 19p12 (rs4324267, P = 1.6 × 10(-5)), 5q14.1 (rs4588572, P-value = 3.36 × 10(-5)), and 15q21.1 (rs4774497, P = 1.08 × 10(-4)). Using the Versatile Gene-Based Association Study to complement the PBAT results, we found clusters of markers located at chromosomes 19p12, 11q25, and 8p23.2 overcome the threshold for GWAS significance (P < 1 × 10(-7)). From this study, new recessive loci implicated in NS CL/P include: B3GAT1, GLB1L2, ZNF431, ZNF714, and CSMD1, even though the functional association with the genesis of NS CL/P remains to be elucidated. These results emphasize the importance of using homogeneous populations, phenotypes, and family structures for GWAS combined with gene-based association analyses, and should encourage. other researchers to evaluate these genes on independent patient samples affected by NS CL/P. Copyright © 2012 Elsevier Masson SAS. All rights reserved.

Citation

Mauricio Camargo, Dora Rivera, Lina Moreno, Andrew C Lidral, Ursula Harper, Marypat Jones, Benjamin D Solomon, Erich Roessler, Jorge I Vélez, Ariel F Martinez, Settara C Chandrasekharappa, Mauricio Arcos-Burgos. GWAS reveals new recessive loci associated with non-syndromic facial clefting. European journal of medical genetics. 2012 Oct;55(10):510-4