Screening and comparison of differentially expressed genes between one MDR-TB strain and the virulent M. tuberculosis H37Rv.

Yunling Zhang, Gaihuan Zheng, Yuting Wang, Junhua Chen, Chaomin Zhu, Ruixi Liu, Zhe Peng, Qizhi Li, Linlin Xing

The Infection and Digestology Department of Affiliated Children's Hospital, PR China. 371414672@qq.com

Gene 2012 Sep 10

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To screen and compare the differentially expressed genes between one MDR-TB strain separated from one child patient and the virulent Mycobacterium tuberculosis H37Rv, suppression subtractive hybridization (SSH) technology was used to build a library of cDNAs that were differentially expressed in the MDR and H37Rv. From this cDNA library, genes that were expressed in the MDR-TB but not in the H37Rv were selected for gene sequencing and homology analysis; 113 positive clones were obtained, their cDNA fragments were sequenced, and homology analysis was performed. Four novel sequences were identified. The results provide a partial list of genes differentially expressed in MDR-TB and four novel genes were found. Identification of these genes may contribute to our understanding of MDR-TB development. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Yunling Zhang, Gaihuan Zheng, Yuting Wang, Junhua Chen, Chaomin Zhu, Ruixi Liu, Zhe Peng, Qizhi Li, Linlin Xing. Screening and comparison of differentially expressed genes between one MDR-TB strain and the virulent M. tuberculosis H37Rv. Gene. 2012 Sep 10;506(1):223-9