Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro.

Peng Xu, Hilal Gul-Uludag, Woon T Ang, Xiaoyan Yang, Min Huang, Leah Marquez-Curtis, Locksley McGann, Anna Janowska-Wieczorek, James Xing, Eric Swanson, Jie Chen

ECERF W6-019, Department of Biomedical Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada. px1@ualberta.ca

Biotechnology letters 2012 Oct

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Low-intensity pulsed ultrasound (LIPUS) stimulated the viability, proliferation and differentiation of hematopoietic stem/progenitor cells (HSPC) from fresh and cryopreserved peripheral blood leukapheresis product, as well as cord blood when applied for 10 min each day for 4 days. Cell viability, proliferation and differentiation were assessed on day 5 by viable cell counting, MTS proliferation assay, flow cytometry, and colony-forming unit assay. LIPUS stimulation: (i) enhanced the proliferation of fresh HSPC and maintained the viability of cryopreserved HSPC in vitro; (ii) did not affect the percentage of CD34(+) and CD14(+) cells; and (iii) enhanced burst-forming unit-erythroid colony formation. Hence, we suggest that this novel LIPUS stimulation approach might enhance the efficacy of clinical transplantation and cellular therapies using HSPC.

Citation

Peng Xu, Hilal Gul-Uludag, Woon T Ang, Xiaoyan Yang, Min Huang, Leah Marquez-Curtis, Locksley McGann, Anna Janowska-Wieczorek, James Xing, Eric Swanson, Jie Chen. Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro. Biotechnology letters. 2012 Oct;34(10):1965-73