Effects of megsin gene transfection on the expressions of MCP-1 and ICAM-1 in mouse glomerular mesangial cells].

To observe the effects of megsin gene transfection on the expressions of monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1(ICAM-1). Mouse glomerular mesangial cells were cultured in high glucose medium, and then cell proliferation was measured by MTT assay at 12, 24 and 48 h respectively. The expressions of megsin, MCP-1 and ICAM-1 in mesangial cells were detected by immunocytochemical staining and Western blotting. The concentration of type IV collagen in the culture supernatant of mesangial cells was measured by ELISA. Under high glucose concentration, the expressions of megsin, MCP-1 and ICAM-1 increased; the concentration of type IV collagen in the cell supernatant was elevated as well; and mesangial cell proliferation was enhanced. After megsin gene transfection, the above changes were more significant, but were abated following megsin shRNA/transfection. Megsin gene can up-regulate the expressions of MCP-1 and ICAM-1, promote mesangial cell proliferation and mesangial extracellular matrix accumulation.

Citation

Jie Liu, Ying Li, Mao-dong Liu, Guang-wei Ren, Ming-ming Wang, Ying-min Li, Bin Cong. Effects of megsin gene transfection on the expressions of MCP-1 and ICAM-1 in mouse glomerular mesangial cells]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 2012 Jul;28(7):702-5

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PMID: 22768859

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