Thyroid hormones are involved in 5'-nucleotidase modulation in soluble fraction of cardiac tissue.

Guilherme Cotomacci, João J F Sarkis, Cristina R Fürstenau, Maria Luiza M Barreto-Chaves

Department of Anatomy, Laboratory of Cell Biology and Functional Anatomy (LBCAF), Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.

Life sciences 2012 Aug 21

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To investigate the role of TH (thyroid hormones) in 5'-nucleotidase activity and expression in cardiac soluble fraction (SF). Male Wistar rats received daily injections of T4 (10, 25 or 50 μg T4/100g body weight) for 14 days to develop a hyperthyroidism condition. Thyroidectomy was performed in other animals to mimic hypothyroidism, and 14 days after surgery they were submitted to TH replacement therapy. T4 reduced the 5'-nucleotidase activity (T4-25, P<0.05 and T4-50, P<0.01) in the SF. Conversely, hypothyroidism significantly increased the 5'-nucleotidase activity in this fraction (P<0.001) and TH replacement therapy reversed the latter result (P<0.001 compared to hypothyroid group). The analysis of protein expression in the SF showed that 5'-nucleotidase was more expressed in hypothyroid than in the control group and that the phosphorylated state of PKC observed in this condition may contribute to a possible mechanism of 5'-nucleotidase modulation by thyroid status. Taken together, these data reveal that TH can influence adenosine production by modulating 5'-nucleotidase activity and expression, which may contribute to the cardioprotective effect and the maintenance of cardiac function under TH privation. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Guilherme Cotomacci, João J F Sarkis, Cristina R Fürstenau, Maria Luiza M Barreto-Chaves. Thyroid hormones are involved in 5'-nucleotidase modulation in soluble fraction of cardiac tissue. Life sciences. 2012 Aug 21;91(3-4):137-42