Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe.

Huping Jiao, Bin Wang, Jian Chen, Dongli Liao, Wenying Li, Cong Yu

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

Chemical communications (Cambridge, England) 2012 Aug 14

filter terms:

A single stranded oligonucleotide could induce aggregation of a perylene probe, the probe's monomer fluorescence was efficiently quenched. However, when the oligonucleotide was 5'-phosphorylated by polynucleotide kinase, it could be very efficiently degraded by lambda exonuclease, probe monomers were released, and a turn on fluorescence signal was detected.

Citation

Huping Jiao, Bin Wang, Jian Chen, Dongli Liao, Wenying Li, Cong Yu. Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe. Chemical communications (Cambridge, England). 2012 Aug 14;48(63):7862-4