Extraction of bacterial RNA from soil: challenges and solutions.

Yong Wang, Masahito Hayatsu, Takeshi Fujii

Microbes and environments 2012

filter terms:

Detection of bacterial gene expression in soil emerged in the early 1990s and provided information on bacterial responses in their original soil environments. As a key procedure in the detection, extraction of bacterial RNA from soil has attracted much interest, and many methods of soil RNA extraction have been reported in the past 20 years. In addition to various RT-PCR-based technologies, new technologies for gene expression analysis, such as microarrays and high-throughput sequencing technologies, have recently been applied to examine bacterial gene expression in soil. These technologies are driving improvements in RNA extraction protocols. In this mini-review, progress in the extraction of bacterial RNA from soil is summarized with emphasis on the major difficulties in the development of methodologies and corresponding strategies to overcome them.

Citation

Yong Wang, Masahito Hayatsu, Takeshi Fujii. Extraction of bacterial RNA from soil: challenges and solutions. Microbes and environments. 2012;27(2):111-21