Gold nanoparticles for immunogold localization of antigens in plant tissues.

Sadaki Yokota

Division of Functional Morphology, Nagasaki International University, Sasebo, Nagasaki, Japan. syokota@niu.ac.jp

Methods in molecular biology (Clifton, N.J.) 2012

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Here we describe postembedding immunoelectron microscopic technique applied to ultrathin sections of plant material. The method relies on the use of gold nanoparticles. The methods include tissue fixation, dehydration, embedding of plant specimens, and staining of semithin and ultrathin sections. The described method is suitable for localization of antigens including proteins (peroxisomal enzymes) and a low molecular substance such as ginsenoide-Rb1.

Citation

Sadaki Yokota. Gold nanoparticles for immunogold localization of antigens in plant tissues. Methods in molecular biology (Clifton, N.J.). 2012;906:57-70