Shewanella frigidimarina microbial fuel cells and the influence of divalent cations on current output.

Lisa A Fitzgerald, Emily R Petersen, Dagmar H Leary, Lloyd J Nadeau, Carissa M Soto, Richard I Ray, Brenda J Little, Bradley R Ringeisen, Glenn R Johnson, Gary J Vora, Justin C Biffinger

Chemistry Division, US Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375, USA.

Biosensors & bioelectronics 2013 Feb 15

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The genes involved in the proposed pathway for Shewanella extracellular electron transfer (EET) are highly conserved. While extensive studies involving EET from a fresh water Shewanella microbe (S. oneidensis MR-1) to soluble and insoluble electron acceptors have been published, only a few reports have examined EET from marine strains of Shewanella. Thus, Shewanella frigidimarina (an isolate from Antarctic Sea ice) was used within miniature microbial fuel cells (mini-MFC) to evaluate potential power output. During the course of this study several distinct differences were observed between S. oneidensis MR-1 and S. frigidimarina under comparable conditions. The maximum power density with S. frigidimarina was observed when the anolyte was half-strength marine broth (1/2 MB) (0.28 μW/cm(2)) compared to Luria-Bertani (LB) (0.07 μW/cm(2)) or a defined growth minimal medium (MM) (0.02 μW/cm(2)). The systematic modification of S. frigidimarina cultured in 1/2 MB and LB with divalent cations shows that a maximum current output can be generated independent of internal ionic ohmic losses and the presence of external mediators. Published by Elsevier B.V.

Citation

Lisa A Fitzgerald, Emily R Petersen, Dagmar H Leary, Lloyd J Nadeau, Carissa M Soto, Richard I Ray, Brenda J Little, Bradley R Ringeisen, Glenn R Johnson, Gary J Vora, Justin C Biffinger. Shewanella frigidimarina microbial fuel cells and the influence of divalent cations on current output. Biosensors & bioelectronics. 2013 Feb 15;40(1):102-9