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Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant.
Harold J Schreier, Wipawee Dejtisakdi, Jose Ortega Escalante, Marina Brailo
Applied and environmental microbiology 2012 OctSizes of these terms reflect their relevance to your search.
A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.
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Harold J Schreier, Wipawee Dejtisakdi, Jose Ortega Escalante, Marina Brailo. Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant. Applied and environmental microbiology. 2012 Oct;78(19):7120-3
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PMID: 22798371
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