Construction of ectoine absorption defective mutant for efficient ectoine production].

To further enhance ectoine (1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) synthesis efficiency. We cloned Halomonas salina DSM 5928(T) teaABC gene of ectoine-specific transporter TeaABC by walking PCR and constructed ectoine absorption defective mutant H. salina DSM 5928(T) (teaABC-) by Red recombination technology. The total concentration of ectoine and productivity in a 10 L fermentor were 9.10 (+/- 0.08) g/L and 9.93 (+/- 0.09) g/L. d. The ectoine absorption defect mutant H. salina DSM 5928(T) (teaABC-) compromised the negative feedback regulation of ectoine synthesis, which can significantly improve the efficiency of ectoine production.

Citation

Rui Xu, Linghua Zhang. Construction of ectoine absorption defective mutant for efficient ectoine production]. Wei sheng wu xue bao = Acta microbiologica Sinica. 2012 May 4;52(5):661-7

Mesh Tags

Substances

PMID: 22803353

search → result