A lateral flow biosensor for detection of single nucleotide polymorphism by circular strand displacement reaction.

Zhuo Xiao, Puchang Lie, Zhiyuan Fang, Luxin Yu, Junhua Chen, Jie Liu, Chenchen Ge, Xuemeng Zhou, Lingwen Zeng

South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China.

Chemical communications (Cambridge, England) 2012 Sep 4

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A lateral flow biosensor for detection of single nucleotide polymorphism based on circular strand displacement reaction (CSDPR) has been developed. Taking advantage of high fidelity of T4 DNA ligase, signal amplification by CSDPR, and the optical properties of gold nanoparticles, this assay has reached a detection limit of 0.01 fM.

Citation

Zhuo Xiao, Puchang Lie, Zhiyuan Fang, Luxin Yu, Junhua Chen, Jie Liu, Chenchen Ge, Xuemeng Zhou, Lingwen Zeng. A lateral flow biosensor for detection of single nucleotide polymorphism by circular strand displacement reaction. Chemical communications (Cambridge, England). 2012 Sep 4;48(68):8547-9