Correlation Engine 2.0
Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

To explore the effects and mechanism of mesangial cell (MC) on proliferation and function of osteoblast in vitro from growth factors. Osteoblast cultured in vitro were divided into normal blood serum group and MC group and cultured supernatant fluid by 10% serum culture medium and 20% MC culture medium. MTF was used to checked the proliferation of osteoblast at the end of 24, 48, 72, 120 h; Expression of BGP and OPN were tested 72 h and 144 h after culture; Expression of IGF-alpha and TGF-beta were tested 144 h after culture. The proliferation of osteoblast in MC group was obviously higher than normal blood serum group in different times (P < 0.05); The level of BGP and OPN of osteoblast was obviously higher than normal blood serum group (11.3%, 16.4%, 55.0% and 39.6%); and the level of IGF-alpha and TGF-beta of osteoblast was obviously higher than normal blood serum group (10.1% and 47.7%). MC can directly act on osteoblast, promote the proliferation and function of osteoblast by promoting secretion of TGF-beta.

Citation

Feng Wu, Ri-yang Lin, Li-qun He. Effects of mesangial cell on proliferation and function of osteoblast in vitro]. Zhongguo gu shang = China journal of orthopaedics and traumatology. 2012 Apr;25(4):324-7

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 22812099

View Full Text