No influence of serotonin levels in foetal bovine sera on radiation-induced bystander effects and genomic instability.

The aim of this study was to contribute to an inter-laboratory investigation within the Non-Targeted Effects of Ionising Radiation Integrated project (NOTE) (2006-2010) to investigate the role of serum serotonin concentration on radiation-induced bystander effects using our successful experimental design. Two sera of high and low serotonin levels were tested alongside standard serum used in our laboratory. Primary Human Fibroblast 19 (HF19) cells were sham/irradiated with 0.5 Gy alpha-particles, in medium supplemented with serum of different levels of serotonin. Filtered medium was transferred to unirradiated HF19 bystander cells. Cells from irradiated and bystander populations were harvested for chromosomal analysis at early and delayed times post-irradiation/media transfer to assess initial damaging effects and induction of delayed chromosomal instability respectively. Chromosomal damage was elevated to significant levels (p = ≤ 0.005) above respective controls in both cell populations in all groups. Induction of delayed chromosomal instability was significantly observed in all groups at delayed time post irradiation/medium transfer. Furthermore, induction of bystander effects at early and delayed times was not significantly different between groups. No effect of serotonin on the induction of either bystander effects of genomic instability was observed using this experimental system.

Citation

Kim L Chapman, Ammar H J Al-Mayah, Deborah A Bowler, Sarah L Irons, Munira A Kadhim. No influence of serotonin levels in foetal bovine sera on radiation-induced bystander effects and genomic instability. International journal of radiation biology. 2012 Oct;88(10):781-5

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PMID: 22812666

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