Towards understanding the phosphorylation code of tau.

We describe our efforts to combine in vitro enzymatic reactions with recombinant kinases to phosphorylate the neuronal tau protein, and NMR spectroscopy to unravel the resulting phosphorylation pattern in both qualitative and quantitative manners. This approach, followed by functional assays with the same samples, gives access to the complex phosphorylation code of tau. As a result, we propose a novel hypothesis for the link between tau (hyper)phosphorylation and aggregation.

Citation

Guy Lippens, Laziza Amniai, Jean-Michel Wieruszeski, Alain Sillen, Arnaud Leroy, Isabelle Landrieu. Towards understanding the phosphorylation code of tau. Biochemical Society transactions. 2012 Aug;40(4):698-703

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PMID: 22817719

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