A rapid and simplified method for protein silver staining in polyacrylamide gels.

Lijing Zhao, Cong Liu, Yinpeng Sun, Liping Ban

College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.

Electrophoresis 2012 Jul

filter terms:

Silver staining is widely used to detect protein in polyacrylamide gels when high sensitivity is required. A simple and rapid protocol for silver staining of proteins following PAGE was developed in the present study. The number of steps was reduced compared to conventional protocol by combining fixing, rinsing, and soaking into a single impregnating step, thus achieving detection of proteins in 20 min. The present method is as sensitive as current protocols with the advantage of saving time and costs. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Lijing Zhao, Cong Liu, Yinpeng Sun, Liping Ban. A rapid and simplified method for protein silver staining in polyacrylamide gels. Electrophoresis. 2012 Jul;33(14):2143-4