Pilt is a coiled-coil domain-containing protein that localizes at the trans-Golgi complex and regulates its structure.

Hideaki Tamaki, Masashi Sanda, Osamu Katsumata, Yoshinobu Hara, Masahiro Fukaya, Hiroyuki Sakagami

Department of Anatomy, Kitasato University School of Medicine, Sagamihara 252-0374, Japan.

FEBS letters 2012 Sep 21

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Protein incorporated later into tight junctions (Pilt), also termed tight junction-associated protein 1 or tight junction protein 4, is a coiled-coil domain-containing protein that was originally identified as a human discs large-interacting protein. In this study, we identified Pilt as an Arf6-binding protein by yeast two-hybrid screening. By immunocytochemical analysis, Pilt was shown to be predominantly localized at the trans-Golgi complex and to exhibit diffuse cytoplasmic distribution in association with endosomes and plasma membrane in NIH3T3 cells. Silencing of endogenous Pilt disrupted the Golgi structure. The present findings suggest the functional involvement of Pilt in the maintenance of the Golgi structure. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Hideaki Tamaki, Masashi Sanda, Osamu Katsumata, Yoshinobu Hara, Masahiro Fukaya, Hiroyuki Sakagami. Pilt is a coiled-coil domain-containing protein that localizes at the trans-Golgi complex and regulates its structure. FEBS letters. 2012 Sep 21;586(19):3064-70