Antioxidant activity of propolis extracts from Serbia: a polarographic approach.

Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L(-1) alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L(-1) H2O2 decrease. Resulting HPEq ranged between 1.71±0.11 and 8.00±0.18 μL. Range of 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093±0.004% to 0.346±0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31±0.05% g GAE, while the extract with the lowest activity contained 1.45±0.02% g GAE. Antioxidant activity, determined by polarographic method, was correlated with DPPH scavenging activity (R2=0.991) and TCP (R2=0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test. Copyright © 2012 Elsevier Ltd. All rights reserved.

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Nebojša I Potkonjak, Dragan S Veselinović, Miroslav M Novaković, Stanislava Ž Gorjanović, Lato L Pezo, Desanka Ž Sužnjević. Antioxidant activity of propolis extracts from Serbia: a polarographic approach. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2012 Oct;50(10):3614-8

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PMID: 22842121

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