Functional expression of trypsin from Streptomyces griseus by Pichia pastoris.

In the present study, the genes encoding trypsinogen and active trypsin from Streptomyces griseus were both cloned and expressed in the methylotrophic yeast Pichia pastoris with the α-factor secretion signal under the control of the alcohol oxidase promoter. The mature trypsin was successfully accumulated extracellularly in soluble form with a maximum amidase activity of 6.6 U ml(-1) (batch cultivation with flask cultivation) or 14.4 U ml(-1) (fed-batch cultivation with a 3-l fermentor). In contrast, the recombinant trypsinogen formed inclusion bodies and no activity was detected. Replacement of the trypsin propeptide Ala-Pro-Asn-Pro confirmed that its physiological function was as a repressor of activity. More importantly, our results proved that the propeptide inhibited the activity of trypsinogen after its successful folding.

Citation

Zhenmin Ling, Tengbo Ma, Jianghua Li, Guocheng Du, Zhen Kang, Jian Chen. Functional expression of trypsin from Streptomyces griseus by Pichia pastoris. Journal of industrial microbiology & biotechnology. 2012 Nov;39(11):1651-62

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PMID: 22842958

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