Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides.

Ana L Moyano, Romina Comín, Aldo A Vilcaes, Samanta C Funes, Germán A Roth, Fernando J Irazoqui, Gustavo A Nores

Departamento de Química Biológica Dr. Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba and CIQUIBIC, CONICET, Córdoba 5000, Argentina.

Glycobiology 2012 Dec

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Immunization of rabbits with bovine brain gangliosides induced an experimental neuropathy, with clinical signs resembling Guillain-Barré syndrome. All the immunized animals developed immunoglobulin G immunoreactivity to GM1 ganglioside. In a few (4 of 27) animals, an additional anti-ganglioside antibody population showing an unusual binding behavior was detected. Enzyme-linked immunosorbent assay and thin-layer chromatography immunostaining analyses showed that the binding of these unusual antibodies required the presence of two co-localized gangliosides. Maximal interaction was observed to a mixture of GM1 and GD1b, but the antibodies also showed "density-dependent" binding to GD1b. The antibodies were purified by affinity chromatography and displayed the ability to target antigens in biological membranes (rat synaptosomes).

Citation

Ana L Moyano, Romina Comín, Aldo A Vilcaes, Samanta C Funes, Germán A Roth, Fernando J Irazoqui, Gustavo A Nores. Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides. Glycobiology. 2012 Dec;22(12):1768-74