Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline.

As-p18 is a fatty-acid-binding protein from the parasitic nematode Ascaris suum. Although it exhibits sequence similarity to mammalian intracellular fatty-acid-binding proteins, it contains features that are unique to nematodes. Crystals were obtained, but initial diffraction data analysis revealed that they were composed of a number of `microdomains'. Interpretable data could only be collected using a microfocus beamline with a beam size of 12 × 8 µm.

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Mads Gabrielsen, Alan Riboldi-Tunnicliffe, Marina Ibáñez-Shimabukuro, Kate Griffiths, Andrew J Roe, Alan Cooper, Brian O Smith, Betina Córsico, Malcolm W Kennedy. Useable diffraction data from a multiple microdomain-containing crystal of Ascaris suum As-p18 fatty-acid-binding protein using a microfocus beamline. Acta crystallographica. Section F, Structural biology and crystallization communications. 2012 Aug 01;68(Pt 8):939-41

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PMID: 22869127

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